Product Name: Cooling agent ws-3
Chemical Name: N-Ethyl-2-(isopropyl)-5-methylcyclohexanecarboxamide
CAS No.: 39711-79-0
MF: C13H25NO
MW: 211.35
Appearance: White crystal powder
Odor: Mild cooling, slight menthol odor (almost odorless)
Detection Method: HPLC
Purity: ≥99%
Melting Point: 87ºC-102ºC
Flash Point: >100℃
Specific Rotation: -49° ~ -54°
Water Solubility: Insoluble
FEMA No.: 3455
EINECS No.: 254-599-0
Shelf Life: 2 years
Package: 1kg per bag with double plastic inside and aluminium foil bag outside, or 25kg per fibre drum with double plastic bag inside, or according to customers’ requirement.
Storage: Store in cool and dry place. Keep away from strong light and heat.
Product Description:
Background:
WS 3 is an agonist of TRPM8 receptor with EC50 value of 3.7 μM [1].
Transient receptor potential cation channel subfamily M member 8 (TRPM8) is a Ca2+– and Na+-permeable ion channel and is activated by cooling agents and cold temperatures. TRPM8 belongs to TRP family and is expressed in sensory neurons.
WS 3 is an agonist of TRPM8 receptor and a cooling agent. In HEK293 cells expressed recombinant mouse TRPM8, WS 3 (30 μM) increased [Ca2+]i. WS 3 exhibited potency with EC50 value of 3.7 μM and induced 86% efficacy of the maximal response to icilin, the most potent agonist [1]. In HEK cells stably expressing either hTRPM8 or hTRPA1, WS 3 activated hTRPM8 and hTRPA1 with EC50 values of 2.2 and 120.6 μM, respectively. WS 3 was efficacious in eliciting cooling sensation [2].
References:
[1]. Behrendt HJ, Germann T, Gillen C, et al. Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay. Br J Pharmacol, 2004, 141(4): 737-745.
[2]. Klein AH, Iodi Carstens M, McCluskey TS, et al. Novel menthol-derived cooling compounds activate primary and second-order trigeminal sensory neurons and modulate lingual thermosensitivity. Chem Senses, 2011, 36(7): 649-658.
Advantages:
Food Grade Cooling Agent ws-3 For Cigarette/Filter Tip/Tobacco/Oral Product
- There is a high cooling effect without other unpleasant feelings such as bitterness, burning, stinging or tingling sensations and can bring more stimulative, fresh and aromatic sensory to
the entire mouth and throat.
- Cooling agent ws-3 can solve the difficulty of developing new products with bad sensation.
- When used with other flavors, it enhances the effects of the flavors. It also can be used with
other cooling agents.
- Cooling agent ws-3 is water-solubility is no more than 0.1%, it’s better than traditional cooling agents, so it can act on taste bud more wallop and has long cooling effect.
Functions:
- Continuous and long lasting cooling and refreshing effect, having no hot, harsh and stinging sensation of Menthol and/or Peppermint.
- Heat-Resistance: Heating under 200oC will not reduce the cooling effect, suitable to use on baking and other high temperature heating process.
- Its cooling intensity can maintain 15-30 minutes, enriching the refreshing of product and having no burning pain, stabbing and numbness, it’s cooler than traditional Menthol products.
- Low dosage: 30-100 mg/kg dosage will give good cooling effect.
- Excellent compatibility with other flavors, it can enhance the effect of the flavors. It also can be combined using with other cooling agents.
Applications:
- Daily use products: toothpaste, oral products, Air Freshener, skin cream, shaving cream, shampoo, sunscreen, shower cream.
- Food products: confectionery products, chocolate, dairy produce, beer, distilled spirit, beverage,
Chewing Gum.
- Medicine: linctus, diminish inflammation ointment, dyspepsia, antipruritic, liniment, oral cavity
acesodyne, heatstroke pill.
- Others: cigarette, filter tip and other tobacco, it also can be used for driving insects.
Usage:
- Soluble in Ethanol/PG firstly, then adding water solution.
- Or dissolved in flavors firstly, then added into your products.
- Mixed with dementholized peppermint oil can increase the fragrance signally.